Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

Protocols that do not use harsh and dangerous chemicals, e.g., phenol, are available online. They produce sufficient quality to enable most routine PCR applications.

Analyze the results of your PCR reaction via gel electrophoresis. GenScript tells you how to design PCR tools, and provides PCR schemes and PCR steps.

This protocol describes the reagents and procedures required to amplify a segment of double-stranded DNA in a chain reaction catalyzed by a thermostable DNA polymerase.

See our reference page on how to design primers for detailed instructions and check out our protocol on plasmid cloning by PCR to learn how to design primers for cloning purposes.

This article provides a detailed summary of a standard PCR protocol, guiding you through the essential steps, critical components, and considerations for successful DNA amplification.

The first protocol outlines how to set-up a PCR reaction using a PCR "Master Mix". The second protocol sets up the PCR reaction using the individual components found in the master mix. Because a …

Use a fine black Sharpie to label six 0.2 ml PCR tubes with the same ID you labeled your DNA Determine how many PCR reactions, and you will make a “Master mix” that will be used for all the …

This protocol is intended for amplification of DNA fragments by PCR. The Thermo Scientific Phusion Plus DNA Polymerase used in this PCR protocol is well-suited for applications such as high-fidelity …

PCR involves a series of temperature cycles that, although once conducted by moving tubes through various water baths, is now controlled automatically by the use of thermal cyclers, or thermocyclers.